Caco-2 and ht29 cells
WebA commercial casein hydrolysate, which contains peptides RYLGY and AYFYPEL as active molecules, has shown antihypertensive effects after acute and long-term administration. … WebThe most physiological relevant ratios are between 90:10 and 75:25 (Caco-2/HT-29MTX). In this range, the best compromise between model response and the presence of mucus …
Caco-2 and ht29 cells
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WebDec 1, 2009 · For adhesion experiments, Caco‐2 and HT29‐MTX cells were cultured separately, or together at 90 : 10, Caco‐2:HT29‐MTX, ratios to mimic the major cell … WebApr 10, 2011 · The HT29 cell line, however, can be treated with methotrexate (MTX) to differentiate the cells into mucin-secreting goblet cells [138,139]. The production of mucus by HT29-MTX cells increase adhesion of bacterial cells relative to Caco-2 or HT29 cells alone [140,141], further supporting the importance of the presence of mucus to bacterial …
WebIn the present study, the cytotoxic response induced by D. salina biomass on the human adenocarcinoma cell lines Caco-2 and HT29 was different. Caco-2 cells were twice as … WebChemical characterization has shown methyl methanethiosulfinate as major sulphurous compounds. A. roseum bulbs essential oil provides interesting antiproliferative activity against two human colonic adenocarcinoma HT29 and CACO2 cell lines in dose-dependent manner with a half-maximal inhibition (IC50) of 4.64 µg/mL and 8.22 µg/mL respectively.
WebCaco-2, HT29, and SW620 cells were grown in Dulbecco’s modified Eagle medium supplemented with 10% heat-inactivated fetal calf serum, 50 mmol/L HEPES, 2 mmol/L glutamine and antibiotics in a water-saturated atmosphere of 90% air and 10% CO,. To determine cytokine WebJun 1, 2010 · Caco-2, HT29: 67, 78, 87 VSL#3: ↑ MUC2, 3, and 5AC expression (no effect on MUC1) HT29: 105 EcN: No change: HT29: 105 VSL#3: No change: mouse: 45 ... Several Lactobacillus species increased mucin expression in the human intestinal cell lines Caco-2 (MUC2) and HT29 (MUC2 and 3), thus blocking pathogenic E. coli invasion and …
WebDec 1, 2009 · For adhesion experiments, Caco‐2 and HT29‐MTX cells were cultured separately, or together at 90 : 10, Caco‐2:HT29‐MTX, ratios to mimic the major cell types in the intestine. Cells were seeded at a density of 50 000 cells cm −2 onto 24‐well plates (Costar). All cultures were grown in DMEM, and culture medium was changed every 2 days.
Web通过将Caco-2细胞单层与特定的内吞作用抑制剂预孵育，研究了细胞摄取的机制。我们的研究结果表明，Caco-2细胞对带负电的Cru / Ca颗粒的吸收比带正电的Cru / Cs颗粒高约3倍。HT29细胞与Caco-2共培养时，它们分泌的粘液对两种颗粒的摄取和转运的影响微不足道。 tiptoe through the tulips wikiWebPrior to the uptake and transport studies, Caco-2, HT-29 and HT29-MTX cell lines, and co-culture combinations thereof, were evaluated for their capacity to replicate a functional … tiptoeing childrenWebMar 6, 2024 · The Caco-2 cellular monolayer is a widely accepted in vitro model to predict human permeability but suffering from several and critical limitations. Therefore, some … tiptoeing icd 10WebSep 21, 2024 · The cell model adopted consisted of co-cultured Caco-2 and HT29-MTX cells, while inflammatory conditions were mimicked through the incubation of epithelial cells with the conditioned medium of activated macrophages (MCM). The epithelial barrier function was monitored through trans-epithelial electrical resistance (TEER), and ROS … tiptoeing lyricsWebIn the case of 5-FU is was reported an EC 50 of 35.83 ± 1.79 mM for Caco-2 and 25.98 ± 3.11 mM for HT29 [65]. Comparing with the three most promising THEDES, 5-FU presents a greater EC 50 ... tiptoeing exerciseWebJul 1, 2014 · In Fig. 1, Caco-2 cells were seeded in 0.33 cm 2 polycarbonate membrane at densities ranging from 5000 to 30,000 cells per well. HT29-MTX were added two days after Caco-2 seeding at a constant density of 10,000 cells per well. In co-cultures, the apparent permeability of rhodamine 123 from basolateral to apical compartments was significantly … tiptoeing definitionWebOct 6, 2024 · Cell Culture. Caco-2 (p44-48) and HT29-MTX cells (p32-40) were cultured in 75-cm 2 2 in 1.0 μm pore size cell culture inserts (PET, Falcon) in a 12 Well Tissue Culture Plate (Falcon). Cocultures were maintained for 21 days to allow Caco-2 cells to differentiate and develop enterocyte-like features. All cultures were kept at 37 °C in 5% CO 2 ... tiptoeing sound effect