Webcoupled to uorescence detection (HPLC/Fluo) for water samples and LC/MS–MS for urine samples. e results obtained for the water samples are pre-sented in Table 1. Limit of quantication (LOQ) was 0.08 Fig. 1 Outline of the protocol design. Details for GLY, glycine and AMPA supplementation are described in the “Materials and methods” section WebJul 17, 2014 · We previously reported an HPLC-fluorescence detection method based on labeling carboxyl groups with a fluorescent 4- N,N -dimethylaminosulfonyl-7- N - (2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED, Figure 1) molecule; the method was validated using a standard of AA ( 11) (Figure 1 ).
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