Phm1 vector
Webb19 juni 2007 · Lane assignments are as follows: ME1 (pHM1), empty vector; ME1 (pthXo6), single pthXo6 gene in pHM1 vector. (B) Northern blot hybridization analysis of … Webb31 okt. 2024 · The pHM1B4 plasmid was transformed into PXO99A competent cells by electroporation. Survival colonies were genotyped by Sanger sequencing of the targeted gene, and then the mutant strains that meet the requirements were selected for …
Phm1 vector
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WebbFor in vitro studies, we utilized the human myometrial cell line PHM1-41, which was obtained from Dr. Barbara Sanborn and derived from term-pregnant human myometrium (patients not in labor) using a protocol that had received prior institutional approval and immortalized using a vector expressing human papillomavirus E6 and E7 proteins . Cells ... http://www.methanotroph.org/wiki/genetics/
WebbAbstract: The promoter region of Ralstonia solanacearum RipAK gene was PCR amplified，and fused with lacZ reporter gene to generate pHM1：PRiPAKLacZ. β … WebbpHM1, M0163 stain containing a blank pHM1 vector, Rif R, Sp R ΔctrA-ctrA, genetic complementary strain of ctrA. M0163 strain containing a recombinant pHM1:: ctrA …
WebbThe recombinant vectors were transformed into the mutant strain to create a complementary strain (pLCxvgA) as described previously (Qian et al., 2005). An over-expression strain of xvgA (pHMxvgA) was also constructed, containing the full-length xvgA gene ligated into pHM1 vector (Davis et al., 1985). Virulence assays WebbLeaves of 4-week-old N. benthamiana were infiltrated with wild-type Pss harbouring the plasmid containing the DSF synthase (pRpfF) or the empty vector (pHM1) alone or co-inoculated with Xcc EPS (xanthan; 0.5mg ml –1). The bacterial population was measured at 0, 24, and 48h post-inoculation from six 1cm 2 leaf disc areas around the ...
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http://www.methanotroph.org/wiki/genetics/ simpson hospital edinburghWebbEvery recombinant vector was linearized with HindIII and cloned into the pHM1 vector for genetic complementation. The hpa1pthXo1 double complementary vectors were constructed using two steps. First, the hpa1 sequence that was linked to its own promoter was cloned into pHM1 between the PstI and KpnI sites. simpson hot chocolate gifhttp://www.biofeng.com/zaiti/qita/pST1.html razer ornata wrist rest replacementWebb1 juni 2024 · In this study, we constructed a protein expression vector pH3-flag based on the backbone of pHM1, a most widely used vector in Xoo strains, especially a model … razer orochi bluetooth laghttp://www.biofeng.com/zaiti/qita/pHEN1.html razer orochi bluetooth latencyrazer orochi bluetooth pairing not workingWebb6 maj 2024 · The 445-bp promoter region of the RSc0454 gene was fused with the LacZ gene in the pLacZ-Basic vector (Clontech) at KpnI-XhoI sites. Next, the promoter region, … razer orochi bluetooth mouse moving