WebA quantitative PCR instrument, StepOnePlus (Applied Biosystems/Life Technologies Co., Carlsbad, CA, USA), was employed to measure cDNA with a Thunderbird Next Sybr qPCR reagent (Toyobo Co.). The DNA sequences of the PCR fragments for target genes were confirmed by Fasmac Co., Ltd. (Atsugi, Kanagawa, Japan). WebSYBR Green™ Realtime PCR Master Mix is applicable for intercalation assay with SYBR ® Green I. SYBR Green™ Realtime PCR Master Mix -Plus- is effective for reducing non-specific amplifications or primer dimers in the intercalation assay. FEATURES Can be used in glass capillary systems (e.g. LightCycler, Roche Molecular Systems, Inc.).
Thunderbird Next Sybr Qpcr Mix Toyobo Bioz
WebTHUNDERBIRD Next SYBR qPCR Mix Pack Size 1.67ml×3 Quantity Submit for Quotation Add To Wishlist * Free Delivery for Orders SGD$250 (excl. GST) & Above. Specifications More … WebThe Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR ® /FAM channel of most real-time qPCR instruments. hof van saksen bungalowpark
広瀬化学薬品株式会社 - 期間: 2024/09/01~2024/03/26 内容:.
WebThe Official Thunderbird Podcast Is Here! Welcome to the debut episode of the Thunderbird podcast, which we’re affectionately calling the ThunderCast! It’s an inside look at the … WebJan 4, 2024 · Gene expression was detected using the THUNDERBIRD® Next SYBR® qPCR Mix (TOYOBO CO., LTD, Tokyo, Japan). Real-time qPCR was performed on a 7500 Fast Real-Time PCR System (Applied BioSystems, CA, USA). All primers were specific for human genes, and the transcript levels were normalized to those of GAPDH ( Supplemental Table … WebExample 1. Detection of influenza virus RNA. Influenza virus RNAs were detected using the Hot Start TTx (RNA) Kit and Tth DNA Polymerase-based real-time PCR reagent. When 1 µL diluted, purified RNA was included in the 20µL reaction solution, the Hot Start TTx (RNA) Kit was more sensitive than the Tth DNA Polymerase-based reagent. Example 2. faruk colakoglu